Both Granzyme A and B are serine proteases that can mediate apoptotic signaling for CTL and NK cells. Both options are synthesized as an inactive proenzyme, and they’re both stored within the cytolytic granules. They’re also released by the effector cells during degradation. Either antibody is useful for localization of the granzyme B-containing granules and to help characterize activated NK and CTL cells.
More Information
Granzyme B is designed for use as a research tool only and shouldn’t be used to diagnose illness or disease in patients. There is no known clone, and it uses a synthetic peptide from the N-terminus of the human antibody by the same name. It uses a Rabbit IgG isotype with an undetermined epitope. Likewise, its molecular weight is 32 kDa.
Applications
Granzyme B is designed for Immunohistochemistry applications. To prepare the specimen, you should use paraffin-embedded or Formalin-fixed tissue that is suitable for use. Your slides should be deparaffinized appropriately, usually with xylene or one of its alternatives.
When you choose the concentrated version of the formula, it is necessary to dilute the antibody using a ratio of 1:100, though this is an estimation. Because it can only be used in IHC applications, there is a pre-diluted form available for purchase, as well.
To retrieve the antigen, you will want to boil the tissue sections in a 10mM citrate buffer with a pH of 6.0 for 10 minutes. Then, cool it to room temperature for 20. After it has cooled the appropriate length of time, you should incubate for 10 more minutes. Make sure to wash the slides and rinse them with PBS-Tween between steps.
The Granzyme B antibody is perfect for those studying NK and CTL cells. Visit Spring Bioscience now to learn more.